Research in Progress

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John Kuriyan's Page at HHMI

Research Publications and Associated Information
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Activation-triggered subunit exchange between CaMKII holoenzymes facilitates the spread of kinase activity


Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1

A structural role for the PHP domain in E. coli DNA polymerase III

A highly efficient peptide substrate for EGFR activates the kinase by inducing aggregation

Structural Basis for Activation of ZAP-70 by Phosphorylation of the SH2-Kinase Linker

Conformational Coupling across the Plasma Membrane in Activation of the EGF Receptor


How a DNA polymerase clamp loader opens a sliding clamp.

A mechanism for tunable autoinhibition in the structure of a human Ca2+/calmodulin-dependent kinase II holoenzyme

The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2


Intersubunit capture of regulatory segments is a component of cooperative CaMKII activation

Role of the histone domain in the autoinhibition and activation of the Ras activator Son of Sevenless

Analysis of the role of PCNA-DNA contacts during clamp loading


Structural analysis of the catalytically inactive kinase domain of the human EGF receptor 3

Stability of an autoinhibitory interface in the structure of the tyrosine kinase ZAP-70 impacts T cell receptor response

The tyrosine kinase Csk dimerizes through its SH3 domain

Mechanism for Activation of the EGF Receptor Catalytic Domain by the Juxtamembrane Segment

The Mechanism of ATP-Dependent Primer-Template Recognition by a Clamp Loader Complex

Equally Potent Inhibition of c-Src and Abl by Compounds that Recognize Inactive Kinase Conformations

The structure of the leukemia drug imatinib bound to human quinone reductase 2 (NQO2)

Differences in Flexibility Underlie Functional Differences in the Ras Activators Son of Sevenless and Ras Guanine Nucleotide Releasing Factor 1


The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase

Membrane-dependent signal integration by the Ras activator Son of sevenless

Structural Basis for the Recognition of c-Src by Its Inactivator Csk


Inhibition of the EGF receptor by binding of MIG6 to an activating kinase domain interface

Structural Basis for the Inhibition of Tyrosine Kinase Activity of ZAP-70

c-Src Binds to the Cancer Drug Imatinib with an Inactive Abl/c-Kit Conformation and a Distributed Thermodynamic Penalty


Protein-protein Interactions in the Allosteric Regulation of Protein Kinases

A Ras-induced Conformational Switch in the Ras Activator Son of Sevenless

Crystal Structure of the Catalytic α Subunit of E. coli Replicative DNA Polymerase III

A Dimeric Kinase Assembly Underlying Autophosphorylation in the p21 Activated Kinases

An Allosteric Mechanism for Activation of the Kinase Domain of Epidermal Growth Factor Receptor

A Src-Like Inactive Conformation in the Abl Tyrosine Kinase Domain

Structure of the Kinase Domain of an Imatinib-Resistant Abl Mutant in Complex with the Aurora Kinase Inhibitor VX-680

Organization of the SH3-SH2 Unit in Active and Inactive Forms of the c-Abl Tyrosine Kinase

Oligomerization states of the association domain and the holoenyzme of Ca2+/CaM kinase II

Crystal structure of a DNA polymerase sliding clamp from a Gram-positive bacterium


Structure of the Autoinhibited Kinase Domain of CaMKII and SAXS Analysis of the Holoenzyme

High yield bacterial expression of active c-Abl and c-Src tyrosine kinases

Computational Docking and Solution X-ray Scattering Predict a Membrane-interacting Role for the Histone Domain of the Ras-activator Son of Sevenless

Out-of-plane motions in open sliding clamps: Molecular dynamics simulations of eukaryotic and archaeal proliferating cell nuclear antigen

Mapping the interaction of DNA with the Escherichia coli DNA polymerase clamp loader complex


Structural analysis of the inactive state of the Escherichia coli DNA polymerase clamp-loader complex

Structural Analysis of Autoinhibition in the Ras Activator Son of Sevenless

Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases

Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex.

Fluorescence Measurements on the E. Coli DNA Polymerase Clamp Loader: Implications for Conformational Changes During ATP and Clamp Binding

Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex


Tandem Histone Folds in the Structure of the N-terminal Segment of the Ras Activator Son of Sevenless

Crystal Structure of a Tetradecameric Assembly of the Association Domain of Ca2+/Calmodulin-Dependent Kinase II

Structural evidence for feedback activation by Ras·GTP of the Ras-specific nucleotide exchange factor SOS




Nucleotide-Induced Conformational Changes in an Isolated Escherichia coli DNA Polymerase III Clamp Loader Subunit


Structural Basis for the Autoinhibition of c-Abl Tyrosine Kinase

A Reinterpretation of the Dimerization Interface of the N-terminal Domains of STATs



Crystal Structures of the Kinase Domain of c-Abl
in Complex with the Small Molecule Inhibitors PD173955 and STI571


TGF-beta Receptor Phosphorylation Control

Processivity Clamp Loaders II: The wrench-clamp assembly

Processivity Clamp Loaders II: The clamp loader

Channel kinase

Hck and Src Dynamics


Abl kinase domain

E. coli primase - catalytic core (2000)


D.Tok (1999)

 Hck-PP1 Complex (1999)           


Cytoplasmic Domain of Type I TGF-beta Receptor complexed with FKBP12


Karyopherin/Importin-alpha Complex

Ras-Sos Complex       Sos: DH-PH Domains



 Polymerase Clamp Loaders

  Src Tyrosine Kinase Hck / (1997)    


Human PCNA Complexed with p21-waf1-cip1

Nef_SH3 Complex




PP-1 Phosphatase


Yeast PCNA  


v-Src SH2 Domain; first SH2-peptide complex

beta-subunit of E. coli DNA Polymerase III - A Sliding DNA Clamp

X-11 PTB Domain Complexed with non-phosphorylated peptides:
Zhang, Z., Lee, C.-H., Mandiyan, V., Borg, J.-P., Margolis, B., Schlessinger, J., and Kuriyan, J. (1997).
Sequence-specific recognition of the internalization motif of the Alzheimer's amyloid precursor protein by the X11 PTB domain.
EMBO J., 16, 6141-6150.

1X11 (PDB file)

1AQC (PDB file)

E. Coli GrpE:DnaK Complex:
Harrison, C. J., Hayer-Hartl, M., Di Liberto, M., Hartl, F.-U., and Kuriyan, J. (1997).
Crystal structure of the nucleotide exchange factor GrpE bound to the ATPase domain of the molecular chaperone DnaK.
Science, 276, 431-435.

List of contacts between DnaK and GrpE

Structure of GrpE:DnaK complex (PDB File)

c-Crk SH3 domain, Lys peptide complex

c-Crk SH3 domain, Arg peptide complex

Paired Homeodomain Dimer on DNA

Syp Phosphatase SH2 Domain:
Syp SH2 domain - complex with PDGFR:1009 peptide
Syp SH2 domain - complex with IRS-1:895 peptide
Syp SH2 domain - complex with PDGFR:740 peptide
Syp SH2 domain - uncomplexed form

DsbA : E. coli periplasmic disulfide bond forming protein

Trypanothione reductase from Crithidia fasciculata

Thioredoxin Reductase, E. coli, Wild Type