Figure 5 - Specificity of DNA-stimulated clamp release. (a) Relative steady-state fluorescence emission spectra obtained with excitation at 460 nm. 500 nM fluorescein attached to D106C delta-A in clamp loader + 4 uM D253C beta labeled with TMR. (b) Clamp release based upon return of donor fluorescence at 515 nm upon addition of 4 uM hairpin DNAs to donor fluorescence values obtained prior to clamp binding by addition of 1 mM ATP. Error bars represent the standard error of at least two measurements.