Figure 3 - DNA-binding affinity for gamma-BCD mutants. (a) Fluorescence anisotropy of 5’-tamra-labeled 40-nucleotide template annealed to 30-nucleotide primer. 100 nM tamra-labeled DNA was excited at 550 nm with emission signal collected at 580 nm in the presence of 1 µM wild-type beta, 1 mM ADP-BeFx (1 mM ADP, 2 mM BeCl, 10 mM NaF). Fits obtained using the equation as described in Materials & Methods. (b) Dissociation constants of gamma-BCD mutants for DNA. Error bars shown are the standard error of the fit.